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Sunday, July 19, 2020 | History

3 edition of Ultrafast photophysics of proteins found in the catalog.

Ultrafast photophysics of proteins

John Elmore Hansen

Ultrafast photophysics of proteins

by John Elmore Hansen

  • 261 Want to read
  • 20 Currently reading

Published .
Written in English


Edition Notes

Statementby John Elmore Hansen.
Classifications
LC ClassificationsMicrofilm 92/491 (Q)
The Physical Object
FormatMicroform
Paginationviii, 207 leaves
Number of Pages207
ID Numbers
Open LibraryOL1388113M
LC Control Number92955242

The primary photoprocess in epicocconone, a protein stain, has ben identified as ultrafast photoisomerization,In its aducts with proteins, this process is not important and the excitation is not of p-p* type. Rather, the flexing of the ring has ben found to take the molecule to a . We use the statistics of photon emission from single molecules to probe the ultrafast dynamics of an unfolded protein via Förster resonance energy transfer. Global reconfiguration of the chain occurs on a time scale of ≈50 ns and slows down concomitant with chain collapse under folding conditions. These diffusive dynamics provide a missing link between the phenomenological chemical kinetics.

Ultrafast Studies of the Photophysics of Cis and Trans States of the Green Fluorescent Protein Chromophore Article in Journal of Physical Chemistry Letters 3(16) .   Photophysics of pyridine. Yang et al. used ultrafast electron diffraction to reveal both the structural and electronic changes that occur when photoexcited pyridine relaxes back to the ground state. The excited state has a distorted (prefulvenic) structure.

  Get this from a library! Ultrafast Phenomena IV: Proceedings of the Fourth International Conference Monterey, California, June , [David H Auston; Kenneth B Eisenthal] -- The motivating idea of the first Topical Meeting on Picosecond Phenomena, which took place at Hilton Head Island in , was to bring together scien tists and engineers in a congenial setting who. To promote photophysics and photochemistry and to foster an interest in the subject in industry, academia and the public. Scope. The traditional fields of photophysics and photochemistry are now the basis for diverse modern-day applications in areas such as solar energy conversion, electronics, sensors, nanotechnology, biology and medicine.


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Ultrafast photophysics of proteins by John Elmore Hansen Download PDF EPUB FB2

The irreversible photoconversion of TV green fluorescent protein (GFP) via decarboxylation is studied under femtosecond excitation using an accumulative product detection method that allows us to measure small conversion efficiencies of down to ΔOD = 10 −7 absorbance change per pulse.

Power studies with and nm pulse excitation reveal that excitation to higher states of Cited by: Cis–trans photoisomerization is proposed as a key process in the photoswitching of some photoactivatable fluorescent proteins.

Here we present ultrafast fluorescence measurements of the model GFP chromophore (HBDI) in the cis state and in a mixture of the cis and trans states.

Our results demonstrate that the mean lifetimes of the cis and trans states are remarkably by: We present a comparative study of the ultrafast photophysics of all-trans retinal in the protonated Schiff base form in solvents with different polarities and -state spectra of retinal in the protonated Schiff base form show large absorption-emission Stokes shifts (– cm −1) for both polar and nonpolar a broadband fluorescence up-conversion Cited by:   In brief, the photophysics leading to the final photoaquated species is complex and involves ultrafast intramolecular relaxation down to the lowest triplet state, photodissociation, and formation of the triplet pentacoordinated species, which then undergoes conformational changes to finally attach a solvent molecule on a timescale of 20 by: 4.

The ultrafast intra-retinal processes but also dielectric retinal-protein interactions receive continued interest [1]. With this background, it is worthwhile understanding the photophysics of the retinylidene chromophore in well-characterized solvents for reference purposes [2, 3].

We present new femtosecond transient-absorption and picosecond fluorescence experiments performed on OBIP, the oxyblepharismin-binding protein believed to trigger the photophobic response of the ciliate Blepharisma japonicum. The formerly identified heterogeneity of the sample is confirmed and rationalized in terms of two independent populations, called rOBIP and nrOBIP.

The rOBIP population. Ultrafast photoreaction dynamics in protein nanospaces (PNS) as revealed by fs fluorescence dynamics studies on photoactive yellow protein (PYP) and related systems Ultrafast photophysics of the protonated Schiff base of retinal in alcohols studied by femtosecond fluorescence up-conversion.

This book reflects the heights of knowledge of. The photophysics of cryptophyte light-harvesting Journal of Photochemistry and Photobiology A: Chemistry () 1–17 The photophysics of cryptophyte light-harvesting Alexander B.

Doust a. Photophysics and photo-transformations, anti-fade and switching buffers, environmental influences. Advanced fluorophore photophysics: metallic environment, plasmonic resonance, nanoantennas. How to label my target in practice: labeling of proteins, DNA, lipids, cellular compartments, membranes.

Halo/Snap/Clip tags, nanobodies, click chemistry. In this work, a series of nine Re(I) diimine dicarbonyl complexes of the general molecular formula cis-[Re(N^N)2(CO)2]+ (N^N are various 2,2′-bipyridine (bpy) or 1,phenanthroline (phen) derivatives) were prepared and spectroscopically investigated to systematically evaluate the photophysical consequences.

Photoisomerization—the twisting of bonds in a molecule in response to absorption of light—is exploited in biology to sense light and can influence the photophysical properties of fluorescent proteins used in imaging applications.

Romei et al. studied this behavior by introducing unnatural amino acids into the photoswitchable green fluorescent protein Dronpa2, thus systematically altering. Book Search tips Selecting this option will search all publications across the Scitation platform Selecting this option will search all publications for the Publisher/Society in context.

Ultrafast THz photophysics of solvent engineered triple-cation halide perovskites Journal of Applied Physics(). A combined theoretical and experimental study of the ultrafast photophysics of Rhodamine B. Molecular Physics: Vol.No. 17, pp. The book untitled Ultrafast photophysics and photochemistry of radical precursors in solution contain a lot of information on this.

The writer explains her idea with easy means. The language is very easy to understand all the people, so do not worry, you can easy to read the idea.

The book. Ultrafast stimulated emission (SE) and photoinduced absorption (PA) measurements were performed on films of a new alkyl-substituted conjugated polymer, BuEH-PPV. The 60 ps SE decay time of BuEH-PPV is significantly longer than that of other conjugated polymers.

The structure and dynamics of the Enhanced Green Fluorescent Protein (EGFP), with the chromophore in the zwitterionic protonation state is analysed. To date there is no direct evidence of its presence within the protein; however, the zwitterionic state of the chromophore may play an important role in the complex photophysics of GFP.

In fact it could be linked to the fluorescence fluctuations. The photophysics of the chromophore of the green fluorescent protein in Aequorea victoria (avGFP) are dominated by an excited state proton transfer reaction.

In contrast the photophysics of the same chromophore in solution are dominated by radiationless decay. Photophysics of Molecular Materials: From Single Molecules to Single Crystals. Editor(s): The book provides a useful introduction to the field for newcomers, and a valid reference for experienced researchers in the field.with particular interest to elementary excitation dynamics in the ultrafast time domain.

The Center for Advanced Photonics Research at Temple University focuses on understanding the photochemistry and photophysics of molecules interacting with ultrafast, ultra-intense laser pulses. “Ultrafast” means laser pulses that are on the order of millionths of a billionth of a second long, with our shortest pulses approaching just 4.

Ultrafast dynamics of epicocconone derivatives synthesized by Prof. Franck's group has been studied in order to understand the photophysics of this class of compounds. Prof. Kotha Our collaboration is on truxane and other fluorescent compounds synthesized in Prof. Kotha's laboratory.

Cis-trans photoisomerization is proposed as a key process in the photoswitching of some photoactivatable fluorescent we present ultrafast fluorescence measurements of the model GFP chromophore (HBDI) in the cis state and in a mixture of the cis and trans states.Fluorescence is a photophysical phenomenon, which obeys basic physical laws.

The fluorescence of the autofluorescent proteins arises on the molecular level from chromophores, which are buried in the protein matrix. The three-dimensional, well-defined architecture of the surrounding is a.

Ultrafast photophysics and photochemistry after excited state intramolecular charge transfer in the liquid phase by Hanna A Ernst (Author) ISBN ISBN Why is ISBN important?

ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book. The digit and digit.